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NETs promote pancreatic cancer migration, invasion and EMT via IL‐1β. A, Real‐time PCR analysis of the mRNA levels of indicated inflammatory cytokines. B. ELISA assay was used to measure IL‐1β levels secreted by different cells. C, Indicated proteins were analysed by Western blot analysis. D, Migratory abilities of BxPC3 and MIA PaCa2 were assessed using the wound healing assay. E, Matrigel invasion assay was carried out to identify the invasive abilities of BxPC3 and MIA PaCa2
