FIGURE 3.

HIF‐1α is essential for the activation of fibroblasts. A, The protein levels of HIF‐1α, COL1A2 and α‐SMA were detected by western blot in MEF cells which were treated with Cocl2 and HIF‐1α inhibitor KC7F2 (10 μmol/L). B, The protein levels of COL1A2 and α‐SMA were determined by western blot in MEF cells which were treated with KC7F2, TGF‐β1 and LLC‐CM. C, The protein levels of HIF‐1α was stably knocked out (KO) by CRISPR‐Cas9 in iMEF cells. D, The mRNA levels of Acta2, Pdgfrα and Pdpn were determined by real‐time PCR in HIF‐1α MOCK (MOCK) cells and HIF‐1α KO (H1‐1) cells (n = 3). E, The protein levels of COL1A2, α‐SMA and HIF‐1α were determined by western blot in MOCK and H1‐1 cells which were treated with TGF‐β1, LLC‐CM and Cocl2. (**P < 0.01, ***P < 0.001)