FIGURE 7.

QKI5 overexpression improves preeclampsia (PE)‐related effects in vivo by regulating NRP1/MMP9 expression. A, Relative mRNA expression of NRP1 and MMP9 determined by qPCR from placental tissues obtained from mice belonging to different groups: The saline‐treated group (normal), the L‐NAME‐treated group (PE), the group with L‐NAME‐treated mice bearing adoptively transferred NC HTR‐8/SVneo cells (PE+NC) and L‐NAME‐treated mice bearing QKI5 expressing HTR‐8/SVneo cells (PE+QKI5). B, Representative Western blotting and densitometric quantification of NRP1 and MMP9 protein expressions relative to the β‐actin loading control. C, Representative immunohistochemistry images and corresponding integrated absorbance values from stained placental tissues indicating proliferation by Ki‐67 staining, and NRP1 and MMP9 expressions. D, The blood pressure indicated by mean arterial pressure in different groups over 18 gestational days. E, Total protein concentration determined in urine samples from day 18.5. Shown are the means ± SD from five mice from each group. Statistical significance was calculated using one‐way analysis of variance. ^** P < .01; ^*** P < .001; ^**** P < .0001 (compared with the non‐targeting group); ^# P < .05; ^## P < .01; ^### P < .001 (compared with the PE+NC group)