FIGURE 4.

Prostaglandin E₃ inhibits inflammation in vivo. C57BL6/J mice were stimulated with LPS to induce acute inflammation. Mice in the control group were injected with saline and those in the LPS‐PGE₃ group were injected with LPS and PGE₃ together. A, After 24 h, mouse peritoneal macrophages (MPMs) were isolated and analysed by flow cytometry. B, ELISA was performed to determine the concentrations of IL‐6 and TNF‐α in both serum and MPM lysates. Graphs represent means ± SD; NS, No significance, *P ≤ .05, ***P ≤ .001