FIGURE 5.

Prostaglandin E₃ suppresses TAM polarization and enhances the phagocytosis of TAM. TAMs were derived from THP‐1 cells co‐cultured with PC3 cells. A, CD206 protein expression on macrophages was analysed by flow cytometry. B, CD206 expression in TAMs following PGE₃ treatment (100 nmol/L). C‐D, TAM cell markers were detected by qPCR compared to M0 cells, and TAM markers were measured following PGE₃ treatment. E, COX2 protein expression following PGE₃ treatment. F, Phagocytosis of TAM detected by Flow cytometry. G, Phagocytosis of TAM detected by Neutral red solution. Graphs represent means ± SD; ***P ≤ .001