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. 2021 May 4;25(12):5691–5706. doi: 10.1111/jcmm.16583

FIGURE 6.

FIGURE 6

KLF15 suppresses MC proliferation by enhancing p53 SUMO1 conjugation. A‐C, HRMCs were transfected with negative control siRNA (SI CON) or SUMO1 siRNA (SI SUMO1) and incubated with PDGF‐BB. A, The expression of SUMO1‐P53 and input (P53) was assessed by IP analysis. B, The expression of KLF15 and SUMO1 was assessed by Western blot analysis. C, Quantitative data. D‐F, HRMCs were transfected with a control plasmid and negative control siRNA (VECTOR + SI CON) or SUMO1 siRNA (VECTOR + SI SUMO1), and KLF15 overexpression plasmid and negative control siRNA (KLF15 + SI CON) or SUMO1 siRNA (KLF15 + SI SUMO1) and incubated with PDGF‐BB. D, The expression of SUMO1‐P53 and input (P53) was assessed by IP analysis. E, The expression of KLF15 and SUMO1 was assessed by Western blot analysis. F, Quantitative data. G‐H, Cell proliferation assay results. *P < .05, n = 3