FIGURE 4.

Wogonin prevents hepatocytes from LPS/TNF‐α‐induced apoptosis. A, qRT‐PCR analysis of TNF‐α, IL‐1β and IL‐6 mRNA levels and (B) immunoblotting analysis of p65 and p‐p65 (Ser536) after LPS/TNF‐α and wogonin treatment with indicated concentrations in AML12 cells. C, Representative flow cytometry images and analysis of cell apoptosis after indicated treatment in AML12 cells. D, Immunoblotting analysis of pro‐apoptotic Bax, Cleaved‐Caspase3, Cleaved‐PARP and anti‐apoptotic Bcl2 expression related to (C). E, Densitometry analysis of Cleaved‐caspase3, Cleaved‐PARP, Bax and Bcl2 related to (D). NC, the negative control group. The results are presented as mean ± SD through at least 3 independent experiments and were analysed by one‐way analysis of variance (ANOVA). *P < .05, **P < .01, ***P < .001