FIGURE 5.

Yin Yang 1 (YY1) was the target gene of miR‐651‐3p in trophoblast cells. A, The Venn diagram showed that four databases identified 88 potential target genes for miR‐651‐3p. B, The binding sites between YY1 and miR‐651‐3p were predicted by miRDB, Mirtarbase, Targetscan, Diana. C, Dual‐luciferase reporter assay performed in 293T cells showed that there was a significant reduction in luciferase activity in cells co‐transfected with miR‐651‐3p and YY1‐WT compared to cells co‐transfected with miR‐651‐3p and YY1‐MUT. D‐G, Transfection of HTR8/SVneo with miR‐651‐3p led to a decrease in the mRNA and protein levels of YY1 while transfection with miR‐651‐3p inhibitor led to an increase in the levels. H, I, The mRNA and protein expression levels of placental YY1 in PE patients were lower than that in the control group. (Data are showed as median and IQR. Statistical analysis was performed with nonparametric test.) J, MiR‐651‐3p was inversely correlated with the expression of lncRNA‐ATB assessed by Pearson analysis (r = −.4398, P <.05). *P <.05, **P <.01