FIGURE 6.

Effect of Yin Yang 1 (YY1) on cell migration, invasion and trophoblast‐endothelial cell interactions networks was mediated by miR‐651‐3p. A‐D, The quantitative real‐time PCR and western blot results showed that pcDNA3.1‐YY1 increased, while siRNA‐YY1 decreased the mRNA and protein expression levels of YY1. E‐H, Co‐transfection experiments of miR‐651‐3p and YY1 was conducted to further verify the direct effects. I, J, Cell migration was detected by wound healing assay. K, L, Cell invasion was measured by transwell assay. M, Si‐YY1 significantly decreased HTR‐8/SVneo cells integration into endothelial cellular networks whereas the effect was reversed by co‐transfection with miR‐651‐3p inhibitor. *P <.05, **P <.01