Fig. 6. Pdx1 promoter and ChIP experiments performed with MIN6-K8 cells.

a Activation of a Pdx1 promoter-luciferase construct (Pdx1-pGL3) in MIN6-K8 cells is significantly reduced after barr1 knockdown. pGL3: empty vector (negative control). b Representative pull-down assay demonstrating the selectivity of the barr1 antibody used for ChIP experiments. Lysates from MIN6-K8 cells were incubated with Sepharose beads coated with barr1 antibody or normal rabbit IgG, followed by elution of captured proteins and Western blotting studies. c, d ChIP experiments were carried with MIN6-K8 cells transfected with either scrambled control or barr1 siRNA, using antibodies against barr1 (c) or p300 (d). The presence of specific Pdx1 promoter sequences in the input DNA recovered from the antibody-bound chromatin segments were analyzed by qPCR (see text for details). Data were normalized to the corresponding input controls. Data were given as means ± s.e.m. from four independent experiments. P values are indicated in the different panels (unpaired two-tailed t-test). Source data are provided as a Source data file.