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. 2021 Jun 7;12(6):587. doi: 10.1038/s41419-021-03858-7

Fig. 5. DGCR5 regulates Mcl-1 expression through interacting with SRSF1.

Fig. 5

A SRSF1 regulated the alternative splicing of Mcl-1 pre-mRNA. B qRT-PCR was showed the Mcl-1L-to-Mcl-1S ratio in ESCC tissues. C Mcl-1L mRNA was obviously increased in TE1 and Kyse170 cells. D The Mcl-1L-to-Mcl-1S ratio was detected when SRSF1 knockdown or overexpressed by qRT-PCR. E Mcl-1 protein level in ESCC cells was investigated when overexpression or knockdown SRSF1 by western blot. F, G Knockdown of DGCR5 inhibited the expression of Mcl-1 while SRSF1 overexpression promoted it at the mRNA and protein level, but knockdown DGCR5 and SRSF1 overexpression could reverse it. *P < 0.05 and **P < 0.01.