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. 2021 Jun 7;12:3406. doi: 10.1038/s41467-021-23494-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Magnetic bead-based affinity isolation of binding partners using His-tagged SARS-CoV-2 spike glycoprotein as a bait for proteins in SARS-CoV-2-positive patient plasma (n = 8). Volcano plot depicting significantly enriched constant chains of immunoglobulins. b Volcano plot depicting significantly enriched non-immunoglobulin proteins (n = 8). c Comparison of SARS-CoV-2 spike glycoprotein pulldown using plasma from COVID-19 ICU patients (n = 8) and non-COVID-19 patients (n = 3). Significance was determined by paired Student’s t tests for (a) and (b) and unpaired Student’s t tests for (c). d LGALS3BP levels across three patient cohorts as determined by DIA-MS or ELISA: control patients before undergoing elective cardiac surgery (n = 30), pre-pandemic sepsis ICU patients (n = 12) and COVID-19 ICU patients (n = 74). Kruskal–Wallis and Dunn’s multiple comparisons tests were used to determine statistical significance. e Volcano plot representing protein changes between baseline plasma samples from patients in ICU with either sepsis (n = 12) or COVID-19 (n = 12). Significance was determined through the Mann–Whitney U test with Benjamini-Hochberg’s FDR correction. f Plasma proteins correlating to LGALS3BP after age and sex corrections in COVID-19 ICU patients (n = 12) are highlighted by a Spearman correlation matrix across the proteomic dataset. Proteins with a Spearman correlation coefficient greater than 0.5 were used for gene ontology pathway enrichment analysis (Supplementary Fig. 10). All statistical analyses are two-tailed.