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. 2021 Jan 18;28(6):1900–1909. doi: 10.1038/s41418-020-00715-6

Fig. 1. mTOR deletion hinders the differentiation of immature NK cells.

Fig. 1

Flow cytometric analysis (A) and the numbers (B) of NK cells (CD3−NK1.1+) in the spleen, bone marrow (BM), lymph nodes (LN), livers, and lungs of WT (n = 7), Mtorfl/fl/CD122Cre/+ (n = 6), and Mtorfl/fl/Ncr1-CreTg (n = 6) mice are illustrated. C The percentage (left) and the number (right) of NK progenitor (CD3−CD122+NK1.1−) in spleen and bone marrow of WT, Mtorfl/fl/CD122Cre/+ and Mtorfl/fl/Ncr1-CreTg mice are illustrated (n = 3/genotype). D Flow cytometry analysis of the CD27 and CD11b expression on gated NK cells from spleen and BM of WT (n = 7), Mtorfl/fl/CD122Cre/+ (n = 5), and Mtorfl/fl/Ncr1-CreTg (n = 4) mice (left) are illustrated. The numbers of each subset were quantified (right). DN (CD27−CD11b−), CD27 SP (CD27+CD11b−), DP (CD27+CD11b+), and CD11b SP (CD27−CD11b+) cells. For the bar graphs, each dot represents one mouse and the data represent at least three independent experiments, and one (C, D) or two-pooled (B) independent experiments are illustrated. Data are indicated as mean ± SD. Statistical significance was calculated using one-way ANOVA. *P < 0.05, **P < 0.01, and ***P < 0.001. ns not significant.