Fig. 2. The induction of E4BP4 by IL-15 requires mTOR.

A Representative overlaid histograms demonstrate the E4BP4 expression level of naïve and IL-15 complex (50 ng/ml) stimulated NK cell subsets in the spleens from WT (n = 6) and Mtor^fl/fl/CD122^Cre/+ (n = 9) mice (left). The mean fluorescence intensity (MFI) was quantified (right). B Representative overlaid histograms demonstrate CD122 expression level (left) of gated NK cell subsets in the spleen from WT (n = 12) and Mtor^fl/fl/CD122^Cre/+ (n = 8) mice. The MFI was quantified (right). C Representative overlaid histograms show the phosphorylation level of STAT5 in naive and IL-15 complex (50 ng/ml) stimulated NK cell subsets in the spleen from WT (n = 4) and Mtor^fl/fl/CD122^Cre/+ (n = 5) mice (left). The MFI was quantified (right). The data represent four independent experiments, and one (A, C) or two-pooled (B) independent experiments are shown. Data are indicated as mean ± SD. Statistical significance was calculated using unpaired Student’s t tests (two tailed). *P < 0.05, **P < 0.01, and ***P < 0.001.