Skip to main content
. 2021 Jan 18;28(6):1900–1909. doi: 10.1038/s41418-020-00715-6

Fig. 3. Ectopic expression of E4BP4 significantly rescues mTOR-deficient NK cell development.

Fig. 3

A, B WT and Mtorfl/fl/CD122Cre/+ mice were treated with 5-FU for 4 days, and bone marrow cells were collected for spin infection with MSCV retrovirus that encoded control GFP or E4BP4. The infected BM cells were then transferred into RAG1−/−γc− mice. After 6 weeks, NK cells (CD3−NK1.1+) that were gated GFP+ cells in the spleen (A) and bone marrow (B) were analyzed by flow cytometry. The representative flow cytometric profiles (left) and the percentage of NK cells (right) are illustrated (A, n = 2–4/genotype; B, n = 6–7/genotype). C The percentages of NK cell subsets in spleen (left) and bone marrow (right) from the indicated mice were quantified (n = 3–7/genotype). D The MFIs of Eomes (left) or CD122 (right) of NK cell subsets in spleen from the indicated mice were quantified (left panel, n = 3–4/genotype; right panel, n = 3–7/genotype). One ((A, D), left panel) or two-pooled ((B–D), right panel) independent experiments are shown. The data represent at least three independent experiments. Data are indicated as mean ± SD. Statistical significance was calculated using two-way ANOVA. *P < 0.05, **P < 0.01, and ***P < 0.001.