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. 2021 Jan 18;28(6):1837–1848. doi: 10.1038/s41418-020-00710-x

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© The Author(s), under exclusive licence to ADMC Associazione Differenziamento e Morte Cellulare 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Western blot analysis of FBXO32 expression after transfection of siRNAs targeting FBXO32 in melanoma cells isolated from patient 2. HSP90 expression was probed as loading control. B Representative image of the effects of FBXO32 downregulation on Boyden migration of melanoma cells from patient 2. Bar = 100 µm. C Quantification of patient 2 melanoma cell migration after FBXO32 downregulation (mean ratio ± SD, n = 3). D Western blot analysis of FBXO32 in SKmel28 cells after lentivirus-mediated transduction. HSP90 expression was probed as loading control. E Effects of FBXO32-forced expression on SKmel28 migration. Bar = 100 µm. F Quantification of SKmel28 migration after FBXO32-forced expression (mean ratio ± SD, n = 3).