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. 2021 Jun 7;12:3402. doi: 10.1038/s41467-021-23644-5

Fig. 4. Matrix degradability regulates vascular lumen formation.

Fig. 4

a Quantification of cell migration speed as a function of hydrogel degradability. Analyzed samples contained 12 mM immobilized CGRGDS and 25.2 mM native collagen-derived degradability (NCD) or high degradability (HD) crosslinker, respectively (n = 3 independent experiments). b Morphology of lumenized sprouts formed inside hydrogels of different degradability. Shown are three representative images each, fixed after 14 days of culture. c Quantification of number of perfusable lumen formed inside hydrogels of different degradability (n = 3 independent experiments). d Quantification of maximum bead perfusion distance inside hydrogels of different degradability, relative to the lumen opening position at parent channel (n = 3 independent experiments). e Quantification of average bead perfusion distance inside hydrogels of different degradability, relative to the lumen opening position at parent channel (n = 3 independent experiments). f Quantification of cell migration speed in the presence of the broad-spectrum MMP inhibitor Marimastat (250 nM) compared with non-treated controls. Analyzed samples contained 12 mM immobilized RGD and 25.2 mM HD crosslinker (n = 3 independent experiments). g Morphology of lumenized sprouts formed inside HD hydrogels in the presence or absence of 250 nM Marimastat. h Quantification of number of perfusable lumens inside hydrogels treated with 250 nM Marimastat relative to non-treated controls (n = 3 independent experiments). i Quantification of maximum bead perfusion distance inside hydrogels treated with 250 nM Marimastat relative to non-treated controls (n = 3 independent experiments). j Quantification of average bead perfusion distance inside hydrogels treated with 250 nM Marimastat relative to non-treated controls (n = 3 independent experiments). Composite fluorescence images of 3D projections showing F-actin (cyan), nuclei (magenta), and 4 μm diameter fluorescent beads (yellow) (scale bar, 100 μm). All data are presented as mean ±s.d., p < 0.05 is considered to be statistically significant (two-tailed unpaired Student’s t test). Source data are provided as a source data file.