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. 2021 Jun 7;12(6):583. doi: 10.1038/s41419-021-03870-x

Fig. 3. HJURP promotes the proliferation of PCa cells in vitro and in vivo.

Fig. 3

A, D Western blotting of HJURP-overexpression (A) and knockdown (D) efficiency. B, E CCK8 assay to measure PCa cells viability after HJURP-overexpression (B) and knockdown (E). C, F Colony-formation assay to measure the cell viability of PCa cells after HJURP overexpression (C) and silencing (F). G EdU assay to measure the effects of HJURP on the proliferative capacity of PCa cells. H Western blotting quantification of KI67 expression after HJURP overexpression or knockdown in PCa cells. I Representative IHC staining of HJURP and KI67 in different Gleason grade PCa tissues from our hospital (n = 131, scale bar: 50 µm; magnification: ×200). J HJURP and KI67 protein expression are highly correlated in PCa (n = 131). K, L HJURP can affect the in vivo growth of PCa cells. M, N Representative IHC staining of HJURP and KI67 in PCa-cell xenograft tissues (scale bar: 50 µm; magnification: ×200).