Skip to main content
. 2021 Jun 7;12(6):583. doi: 10.1038/s41419-021-03870-x

Fig. 4. HJURP inhibits CDKN1A to promote the G1/S phase transition.

Fig. 4

A Correlation analysis showed that 119 genes were highly correlated with HJURP (r ≥ 0.06, P < 0.05). B KEGG enrichment analysis of HJURP and its highly correlated genes. C, D Flow cytometry used to measure the proportion of cells in various phases of the cell cycle in HJURP-overexpression (C) and -knockdown (D). E Western blotting quantified CDKN1A expression after HJURP-overexpression or -knockdown in PCa cells. F Western blotting results of CDKN1A knockdown efficiency. G–K CDKN1A knockdown significantly reversed changes in clonogenic potential (G), cell viability (H), proliferative capacity (I), and cell cycle (J, K) in PC3 cells caused by HJURP inhibition. L Representative IHC staining of HJURP and CDKN1A in different Gleason grade PCa tissues from our hospital (n = 131, scale bar: 50 µm; magnification: ×200). M HJURP and CDKN1A protein expression are highly negatively correlated in PCa (n = 131).