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. 2021 Mar 24;124(12):2004–2016. doi: 10.1038/s41416-021-01336-7

Fig. 4. Radezolid represses RRBP1 protein expression and regulate YAP1 target genes in chemo-resistant OSCC.

Fig. 4

a Structure of Radezolid. b Molecular docking of RRBP1 with Radezolid compounds carried out in Glide and representation in surface view receptor of drug interaction. c Ribbon model structure of RRBP1 showing the hydrogen bonding interaction with Radezolid at PRO115, ARG101 and GLU-76. Hydrogen bonds are shown as dashed lines. d Active site residues within 5 Angstrom of RRBP1 and Radezolid interactions. e Ligand plot showing interaction of the Radezolid interaction with different residues of RRBP1 within 5 angstrom distance. f Indicated chemo-resistant OSCC cells were treated with Radezolid in a dose dependent manner for 48 h, after which lysates were isolated to perform immunoblotting against β-actin and RRBP1. g H357CisR cells were treated with Radezolid in a dose dependent manner for 48 h, after which lysates were isolated to perform immunoblotting against indicated antibody. h H357CisR cells were treated with Radezolid in a dose dependent manner for 48 h, after qRT-PCR was performed to determine the relative mRNA expression (fold change) of indicated genes. GAPDH was used as an internal control.