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. 2021 Jun 7;12:3356. doi: 10.1038/s41467-021-23612-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Confocal fluorescence images of mVenus-cyclin D1 following photobleaching and dissociation from CDK4-mTurquoise-Δ50 lamin A complexes in cells depleted of p21 and p27 by siRNA-mediated co-knockdown. b Comparison of cyclin D1-CDK4 (n = 26 cells, 3 biological replicates), cyclin D1-CDK6 (n = 14 cells, 2 biological replicates) and cyclin E1-CDK2 (n = 18 cells, 3 biological replicates) mVenus-cyclin FRAP timecourses in cells depleted of p21 and p27. c mVenus-cyclin D1 FRAP timecourses in CDK4-mTurquoise-Δ50 lamin A complexes comparing cells depleted of p21 and p27 (n = 26, data from (b)) to cells co-transfected with mRuby3-p21 (n = 14 cells, 3 biological replicates) or mRuby3-p27 (n = 10 cells, 2 biological replicates). d mVenus-cyclin D1 FRAP timecourses in CDK6-mTurquoise-Δ50 lamin A complexes comparing cells depleted of p21 and p27 (n = 14; data (b)) to cells co-transfected with mRuby3-p21 (n = 15 cells, 3 biological replicates) or mRuby3-p27 (n = 13 cells, 2 biological replicates). e mRuby3-p21 (n = 14 cells, 3 biological replicates) or mRuby3-p27 (n = 14 cells, 3 biological replicates) FRAP timecourses in mVenus-cyclin D1 and CDK4-mTurquoise-Δ50 lamin A complexes. f mRuby3-p21 (n = 10 cells, 3 biological replicates) or mRuby3-p27 (n = 11 cells, 3 biological replicates) FRAP timecourses in mVenus-cyclin D1 and CDK6-mTurquoise-Δ50 lamin A complexes. FRAP timecourses show mean ± s.d. Source data are provided as a Source data file.