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. 2021 Jun 7;12:3338. doi: 10.1038/s41467-021-23684-x

Fig. 2. Cross-linking and mass spectrometry of TFIIH/Rad4–Rad23–Rad33/DNA.

Fig. 2

A Non-redundant cross-links identified for TFIIH/Rad4–Rad23–Rad33 on −24/+24 AAF-DNA as a network plot. Intra-subunit cross-links are shown in blue while inter-protein cross-links are shown in gray between TFIIH subunits and in green within Rad4–Rad23–Rad33 and between Rad4–Rad23–Rad33 and TFIIH. B Cross-links were validated by mapping them on the atomic model of TFIIH in this study. 93% of cross-links were consistent with the 40 Å upper limit. C Current model of the Tfb1 PH domain in TFIIH/Rad4–Rad23–Rad33/DNA complex. Of 16 unique cross-links between the PH domain and the other domains of Tfb1, 15 cross-links had Cα-Cα distances less than 40 Å (blue dotted lines), and 1 cross-link exceed 40 Å (red dotted line).The N-terminal end of the TGD (R129) and the C-terminal end of N-Rad4 (V104) are connected by a green dashed line.