Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 18;28(6):1849–1864. doi: 10.1038/s41418-020-00712-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — A Images every 20 min of EGFP-Lamin A positive post-confluent REKs. B Cross-sectional area EGFP-Lamin A positive nuclei over time. C Kymographs (yt projection) of a EGFP-Lamin A positive nucleus over time. D Xz projections of a EGFP-Lamin A positive nucleus. Labelled with nuclear volume. E Xy and xz projections of confocal z-sections of post-confluent REKs stained for Lamin A and Lamin B1. Scale bar = 10 µm. F Cross-sectional area of basal and suprabasal nuclei in post-confluent REKs. Two independent experiments, Welch’s t test, ****p ≤ 0.0001. Intensity of DAPI (G), Lamin A (H) and Lamin B1 (I) staining in basal and suprabasal nuclei in post-confluent REK cultures. Welch’s t test, **p ≤ 0.01, ****p ≤ 0.0001. Representative of two independent experiments. J Images every 15 min of Histone H2B-mCherry positive post-confluent REKs. K Cross-sectional area over time in 45 Histone H2B-mCherry positive nuclei greater than 80 µm². L Kymograph (yt projection) of Histone H2B-mCherry over time; shrunken nucleus (top panels) and a nucleus that does not shrink (bottom panels). M Xz projections of Histone H2B-mCherry positive nuclei labelled with nuclear volume. N Histone H2B-mCherry positive nuclear volume before and after shrinkage. Wilcoxon test, *p ≤ 0.05.