Figure 5.

TUNEL assay in CisPt treated cells. HepG2 and HK-2 cells were treated with CisPt (50 and 100 µM) for 6, 24 and 48 h. Then, the cells and nuclei were visualized using phase contrast (left, magnification 400 ×), TUNEL (FITC 480/30 nm; middle) and Hoechst 33258 stainings (DAPI 375/28 nm; right). Scales correspond to 25 µm.