Table 2.
Comparison of assays detecting pro-apoptotic changes.
| H33258 | Caspases | TUNEL | fPARP-1 | DNA ladder | |
|---|---|---|---|---|---|
| 6 h | |||||
| HepG2 | + | + | +/− | − | − |
| HK-2 | − | ++ | − | + | − |
| 24 h | |||||
| HepG2 | ++ | ++ | ++ | ++ | − |
| HK-2 | ++ | ++ | ++ | ++ | ++ |
| 48 h | |||||
| HepG2 | ++ | ++ | ++ | ++ | ++ |
| HK-2 | ++ | − | ++ | − | ++ |
HepG2 and HK-2 cells were treated with 100 µM CisPt for 6, 24 and 48 h. After treatment, the nuclear changes were detected using five methods, i.e. developed spectrofluorometric assay using Hoechst 33258 (= H33258), caspase 3/7 activity, TUNEL assay, protein expression of PARP-1 fragment (fPARP-1) and DNA ladder. (−, negative; +/−, moderately positive; +, positive; ++, strongly positive).