Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 7;12:3386. doi: 10.1038/s41467-021-23571-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a–f Histological analysis of 3.5 dpc uteri from control (a, c, e) and Smad1/5 cKO (b, d, f) mice was performed by staining with Ki67 (a–b), MUC1 (c–d), or progesterone receptor (PR) (e–f). g qPCR analysis from the endometrial epithelium of control (blue bars) or Smad1/5 cKO (red bars) mice. Paired, two-tailed, t test, mean ± SEM, *p < 0.05, **p < 0.001, ***P < 0.0001. h–i Whole uteri of control (h) and Smad1/5 cKO (i) mice isolated at 4.5 dpc after injection with Chicago Sky Blue dye; implantation sites in the control mice can be visualized as blue bands (indicated by black arrows). Size bar = 1 cm. j–k H&E-stained cross-sections from 4.5 dpc control (j) and Smad1/5 cKO (k) uteri. e=embryo. l–o IHC of FOXO1 i–m and progesterone receptor (PR) n–o in 4.5 dpc uterine cross-sections of control (l–n) and Smad1/5 cKO (m–o) mice. Arrows in l, m indicate the nuclear staining of FOXO1 in the controls (l) and cytoplasmic FOXO1 staining in the Smad1/5 cKO mice (m) E embryo. Representative image of the embryo, observed in at least three specimens from different mice. p qPCR analysis of implantation-related markers in control (blue bars, n = 5) and Smad1/5 cKO (red bars, n = 5) uterine tissues collected at 4.5 dpc. Histology and qPCR analyses were performed in at least three samples of each genotype. Histograms represent mean ± SEM. Paired, two-tailed, t test, *p < 0.033, **p < 0.002, ***P < 0.001. q–u Analysis of decidualization reveals that compared with controls, Smad1/5 cKO cannot respond to the artificial induction of decidualization. Gross images of control (q) and Smad1/5 cKO (r) uteri, (D, decidual horn; N, non-decidual horn; indicated by white arrows). s–t H&E stains of uterine cross-sections of the decidual horns of control (s) and Smad1/5 cKO (t) mice. Yellow arrows in (s) indicate decidualized cells. u Gene expression analysis by qPCR of control (blue bars, n = 6) and Smad1/5 cKO (red bars, n = 6) uterine tissues that received the decidual stimulus. Histology and qPCR analyses were performed in at least three samples of each genotype. Histograms represent mean ± SEM. Paired, two-tailed, t test, *p < 0.033, **p < 0.002, ***P < 0.001.