Fig. 5. Cd36ΔLEC mice have more discontinuity of LEC VE-cadherin junctions and leaky mesenteric lymphatic vessels.
Representative images of whole-mount VE-cadherin (green) immunohistochemistry in (a) lacteals and (b) submucosa collecting vessels in 20-week-old Cd36ΔLEC and control mice (n = 5). TdTomato (TdTom, red) identifies PROX1 positive cells. Images were acquired in tiled scans using a Zeiss LSM 880 Airyscan Confocal Microscope, ×40 objective. Scale bar is 5 μm. c Fluorescent long-chain fatty acid tracer BODIPY C16 was administered intragastrically and integrity and transport function of lymphatic vessels recorded using a stereomicroscope. Scale bars: 500 μm. Right panel shows high-magnification image of the area outlined by the white dashed box (Scale bar: 200 μm). Images are representative of two independent experiments with n representing the number of mice per group.