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. 2021 Jun 7;11:11949. doi: 10.1038/s41598-021-90570-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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ALDH^high cells show enhanced proliferation. (A) Top, Flow diagram showing the gating of ALDH^high and ALDH^low HEC1A cells. Bottom, The proliferation of sorted populations was evaluated over 5 days. Each sample was analyzed in triplicate with data shown as the mean ± SEM. The results shown are representative of three experiments. Two way ANOVA, p = 0.0006. (B) ALDH^high and ALDH^low HEC1B cells were sorted as above (top panel), with cellular proliferation analyzed over 5 days (bottom panel). Each sample was analyzed in triplicate with data shown as the mean ± SEM. The results shown are representative of three experiments. Two-way ANOVA, p = 0.0012.