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. 2021 Jun 7;11:11949. doi: 10.1038/s41598-021-90570-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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ALDH1 is expressed within the basal layer of the endometrium. (A) Representative cores representing the stratum basalis (A1) compared to proliferative (A2) or secretory (A3) endometrium. All images are 100X. The percentage of glands with positive expression were scored. In benign endometrial tissue, (B) Cytoplasmic ALDH1 expression was observed in endometrial glands of the stratum basalis (26.76 ± 3.24, n = 116 cores) compared to both proliferative and secretory endometrium (Kruskal–Wallis p value = 1.145e−14). Significantly lower expression of ALDH1 was observed in the proliferative phase (6.15 ± 1.17, n = 264 cores) and in the secretory phase (9.08 ± 2.43, n = 104).