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. 2021 Jan 18;28(6):1880–1899. doi: 10.1038/s41418-020-00714-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — H3K36me2 levels in epWAT (A) and scWAT (B) of mice fed ND or HFD for 8 weeks (n = 4/group). Flow cytometry analysis of H3K36me2 status in CD45⁺ cells from epWAT (C) and scWAT (D) of mice fed with ND or HFD for 8 weeks. Left: a representative flow cytometry data; right: quantitative data from all mice analyzed (n = 5/group). Representative FACS plots showing the proportion of F4/80⁺ cells gated on CD45⁺ cells from epWAT (E) and scWAT (F) of mice fed with ND or HFD for 8 weeks. Representative FACS plots and quantitative data of H3K36me2 levels in macrophages from epWAT (G) and scWAT (H) of mice after 8 weeks of HFD or ND feeding (n = 5/group). I Western blot analysis of H3K36me2 levels in CD45⁺ cells isolated from scWAT of controls (n = 4) and obese subjects (n = 4). J Representative immunostaining results for CD68 and H3K36me2 in scWAT sections from controls and obese subjects. Scale bars: 50 μm. Original magnification: ×400. Values are expressed as mean ± SEM, and unpaired Student’s t test was employed for data analysis. *P < 0.05; **P < 0.01.