FIGURE 6.

Enhancement of PCV3 replication by NPM1 overexpression in lentivirus-transduced PK15 cells. (A) Lentiviruses were packed in HEK293FT cells, and the lentiviruses of NPM1-GFP and GFP transduced into PK15 cells were examined by microscopy. (B) The viability of lentivirus-transduced PK15 cells was analyzed by CCK-8 assay at 48, 72, and 96 h post-transduction. (C) Lentivirus-transduced PK15 cells and no-transduced PK15 cells were infected with PCV3. The infected cells were harvested at 48, 72, and 96 h post-infection. Western blotting was used to examine the expression levels of Cap, NPM1-GFP, GFP, and β-actin. (D) The copy numbers of viral DNA were determined by qPCR. Data represent the mean ± standard deviation (SD) of three independent experiments (**P < 0.01; NS, not significant).