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. 2021 May 25;15:640709. doi: 10.3389/fncel.2021.640709

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Copyright © 2021 Mehboob, Marchenkova, van den Maagdenberg and Nistri.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Co-expression of Na_V1.7 α1 subunit and P2X3R in WT and R192Q KI cultures. (A) Representative examples of Na_V1.7 α1–P2X3 immunostaining in WT and R192Q KI mouse TG cultures. Nuclei are visualized with DAPI (blue); scale bar = 20 μm. Note the extensive co-staining of Na_V1.7 α1 (red) and P2X3 (green) protein in both WT and KI samples. (B) Histograms represent percentage co-expression of Na_V1.7 α1–P2X3 proteins in WT and KI cultures (p = 0.95, Mann–Whitney test; n = 3 experiments). (C) The histograms quantify the cell diameter distribution of Na_V1.7 α1–P2X3R immunofluorescence in different subgroups in WT and KI cultures.