Figure 6.
Overexpression of Mettl14 rescued the anomaly of neurite outgrowth and post-synaptic formation in primary neurons from Arhgef2−/− mice (at E13.5)
(A) The length of neurites and the number of branches of Zsgreen-positive cells was presented by using ImageJ combined with NeuronJ and Sholl Analysis plugin. Arhgef2 deficiency reduced the longest length of neurites and branch number. Cells were seeded at a density of 2.5 × 104/cm2 and were observed at day 3 after transfection. Scale bar, 25 μm.
(B) After transfection with HBLV-m-Mettl14-Zsgreen for 14 days, the expression of Psd-95 in primary matured neurons was measured by western blot analysis (left). Cells were seeded at a density of 1 × 105/cm2 and were observed at day 14 after transfection. Data are presented as means ± SD from three independent experiments (right), n = 3 per genotype, ∗∗p < 0.01, one-way ANOVA. Data are represented as mean ± SD.
(C) Immunostaining of Psd-95 in primary neurons. Psd-95 (marker for post-synapse, red), Zsgreen: green. Scale bar, 2.5 μm.