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. 2021 May 24;24(6):102645. doi: 10.1016/j.isci.2021.102645

Figure 7.

Figure 7

Overexpression of Npdc1 and Cend1 rescued the anomaly of neurogenesis in primary NPCs from Arhgef2−/− mice (at E13.5)

(A) Images of neuro-spheres showing a significant decrease size of spheres in Arhgef2-knockout NPCs after transfection with HBLV-m-Npdc1/Zsgreen and HBLV-m-Cend1/Zsgreen, respectively. Green: Zsgreen. Scale bar, 100 μm, n = 5 per genotype (2 measurements for each group), ∗∗p < 0.01, ∗∗∗p < 0.001, one-way ANOVA.

(B) Both Npdc1 and Cend1 promoted the generation of βIII-tubulin+ cells (early neuron) and decreased βIII-tubulin-Ki67+ proliferating cells (precursors). Cells were seeded at a density of 5 × 104/cm2 and were observed at day 4 after transfection. βIII-tubulin, marker for early neuron, green; Ki67, marker for proliferating cells, red; DAPI, blue. Scale bar, 50 μm. n = 6 per genotype, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, one-way ANOVA. Data are represented as mean ± SD.