Figure 8.
Overexpression of Npdc1 and Cend1 rescued the anomaly of neurite outgrowth in primary neurons from Arhgef2−/− mice (at E13.5)
(A) Measurement of the length of neurites and the number of branches of Zsgreen-positive cells. Cells were seeded at a density of 2.5 × 104/cm2 and were observed at day 3 after transfection. Scale bar, 25 μm.
(B) After transfection with HBLV-m-Npdc1/Zsgreen and HBLV-m-Cend1/Zsgreen for 14 days, respectively, the expression of Psd-95 in primary matured neurons was measured by western blot analysis. Cells were seeded at a density of 1 × 105/cm2 and were observed at day 14 after transfection. n = 4 per genotype, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, one-way ANOVA. Data are represented as mean ± SD.