FIGURE 9.

SIPR1 serves as a target of miR-181b-5p. (A) After 48 h of transfection with the indicated construct (each at 1 μg) and/or mimic (each at 10 nM), luciferase activity assays were performed. ***, p < 0.001. Average values of three independent experiments are shown. (B) Immunoblot was performed. Representative blots of three independent experiments are shown. (C) Cytoplasmic localization of S1PR1 is shown. (D) The indicated cancer cells were treated with or without rapamycin (5 μM) or CQ (100 μM) for 24 h. Representative blots of three independent experiments are shown. (E) After 48 h of transfection, immunoblot was performed. Representative blots of three independent experiments are shown. (F) After 48 h of transfection, qRT-PCR and immunoblot were performed. ***, p < 0.001. Average values of three independent experiments are shown. (G) After 48 h of transfection, qRT-PCR and immunoblot were performed. ***, p < 0.001. Average values of three independent experiments are shown. (H) ChIP assays were performed as described.