Table 1.
Epitopes selected for validation (ELISA)
| Cluster # | Synthetic peptide sequence | Antigen (genbank ID) | Validation | Selection criteria | Number of peptides in the cluster | Abundancy (%)a |
|---|---|---|---|---|---|---|
| 0 | GGFGSATTTSTPAAGGFGSAAHTSTPAVG | Hypothetical protein (RNE99043) | ELISA (+) | Mild cardiopathic | 4794 | 14 |
| 1 | FGQAAAGDKPPLFGQAAAGDKPSL | Surface antigen 2 (PWU84979) | ELISA (+) | Asymptomatic and mild cardiopathic | 3560 | 15(A)/8.1 (MC) |
| 2 | YKRALPQEEEEDVGPRHVDPDHFRSTT | microtubule-associated protein (RNF14378) | ELISA (+) | Asymptomatic and mild cardiopathic | 1481 | 2.9 (A)/8.5 (MC) |
| 3 | NAGSHELLGTEMPVSGEHFPPNIDSPL | Trans-sialidase (EKG04410) | (−) | Asymptomic & mild cardiopathic | 1300 | 1.1 (A)/4.8 (MC) |
| 7 | PPHTRRVTVRCGPPSCADE | Hypothetical protein (PBJ77896) | Phage-ELISA (+) | Severe cardiopathic | 587 | 40/82 |
| 8 | PSPSAAQHSLPRRHPSPSAAQHSLPRRH | Hypothetical protein (PWV06054) | (−) | Control | 431 | 1.5/0.5 |
| 9 | KVAEAEKQRAAEATKVAEAEKQRAA | Flagellar repetitive antigen (AAA30177) | ELISA (+) | Tandem repeats and mild cardiopathic | 427 | 3.8 |
| 10 | APAKAAAAPAKAAAAPAKAAAAPA | 60S ribosomal protein L19 (PBJ71794) | ELISA (+) | Tandem repeats and asymptomatic | 346 | 1.2/1.0 |
| 12 | AAKTAMGEAGGRSWSNVVKSPHSPR | Hypothetical protein (EKG07867) | ELISA (+) | Mild cardiopathic | 271 | 4.4 |
| 15-2a | PCRGLNYRFPVECGDVLFLGSDGVF | Protein phosphatase 2C (RNF23118) | ELISA (+) | Asymptomatic | 217 | 4.2 |
| 16 | PAAKPAAKPAAKAPAPKA | Putative RNA binding protein (EKF98911) | ELISA (+) | Mild cardiopathic | 202 | 1.9 |
| 19 | SSLAGSDGVGLAGGASSIESFEGLP | Hypothetical protein (EKG03457) | ELISA (+) | Asymptomatic | 168 | 1.1 |
| 24 | AATEKRQSVNNYTTPGDSDGSTAVS | MASP (PBJ79554) | ELISA (+) | Mild cardiopathic | 106 | 1.6 |
| 26 | IEEVRGAAPLGKYALVNTLEGSDG | Hypothetical protein (RNE99359) | (−) | Asymptomatic | 100 | 2.5 |
| 32-3a | PEQEKIPEMVSLIFFGSDGVNLEGT | Trans-sialidase (PWU85340) | ELISA (+) | Asymptomatic | 84 | 0.7 |
| 48-3a | TSFGSDNEKLAGSEGIFGSTSSFST | Hypothetical protein (ESS63713) | ELISA (+) | Asymptomatic | 53 | 0.3 |
| 49 | LADELEQKAAENERLADELEQKAAENER | Flagellar attachment zone protein 1 (PWU93573) | (−) | Tandem repeats and mild cardiopathic | 51 | 0.02 |
| 53 | YIDGKSLGEEEVPLTGEKPLELF | Trans-sialidase (PBJ76118) | ELISA (+) | Mild cardiopathic | 45 | 0.5 |
| 92 | ELQEERRAVARAEVELKKRLQ | Hypothetical protein (PWU83017) | (−) | Severe cardiopathic | 25 | 0.4 |
| 233 | PHGTQRRVATRVEAV | Phospholipase A2-like (EKF31673) | (−) | Severe cardiopathic | 10 | 0.4 |
| 415 | ERRQLLERDPRRNAREIAALEESMN | Calpain-like cysteine peptidase (PBJ79197) | ELISA (+) | Mild cardiopathic | 6 | 10−5 |
| 546 | PAEDVQELVAPAEDVQELVAPAEDV | Hypothetical protein (PWU87792) | (−) | Tandem repeats and severe cardiopathic | 5 | 10−5 |
Synthetic peptides were designed for 20 antigens selected based on their frequency among the different groups of patients and number of peptides in the cluster (A = asymptomatic; MC = mild cardiopathic patient).
a
Overlapping peptides were designed and tested. Only reactive peptides are shown.