REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-Human IgG (γ-chain specific)−Peroxidase antibody produced in goat	Sigma-Aldrich	Cat#A6029; RRID AB_258272
IRDye® 680LT Goat anti-Rabbit IgG Secondary Antibody	Li-Cor	Cat#926-68021; RRID AB_2713919
Anti-fd Bacteriophage antibody produced in rabbit		Cat#B7786; RRID AB_258631
Bacterial and virus strains
M13KO7 Helper Phage	New England Biolabs	Cat#N0315S
E. coli TG1	Lucigen	Cat#60502
E. coli DH10B	Invitrogen	Cat#18297010
Biological samples
Sera from chagasic and healthy donors	University of Sao Paulo Medical School	N/A
Chemicals, peptides, and recombinant proteins
Synthetic peptide for epitope validation in ELISA	Chinese Peptide Company	N/A
Experimental models: organisms/strains
Trypanosoma cruzi Sylvio strain	Gift from Dr. Bianca Zingales (Chemistry Institute, University of Sao Paulo, Brazil) http://iq.usp.br/portaliqusp/?q=en	N/A
Oligonucleotides
Illumina sequencing oligonucleotides (Sequences at Supplementary Data Table S4)	Exxtend (Brazil)	N/A
Recombinant DNA
pG8SAET plasmid	Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala	GenBank: AF130864.1
gPhage library	This paper	N/A
Software and algorithms
BLAST	https://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/	v 2.6.0
PEAR	https://cme.h-its.org/exelixis/web/software/pear/doc.html	v 0.9.10
MAFFT	https://mafft.cbrc.jp/alignment/software/	v 7.307
Jalview	https://www.jalview.org	v 2.11.0
MUSCLE	https://www.ebi.ac.uk/Tools/msa/muscle/	3.8.31
Chimera	https://www.cgl.ucsf.edu/chimera/	V 1.11
XSTREAM	https://amnewmanlab.stanford.edu/xstream/	v 1.73
FuzzyWuzzy	https://github.com/seatgeek/fuzzywuzzy	v 0.8
HMMER	http://hmmer.org/download.html	3.1b2
Sequence analysis script	This article	Data S3
Clustering script	This article	Data S3