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. 2021 May 25;12:670497. doi: 10.3389/fpls.2021.670497

Figure 4.

Figure 4

GA2ox4 is downregulated in MADS12 overexpressing lines during bud dormancy release. (A) qRT-PCR analysis of FT1, GA2ox3, GA2ox4, and GA2ox5 genes in ecodormant MADS12 overexpressing OE3 and OE5 and wild-type (WT) apices collected after 5 days in long day (LD) and 22°C treatment. Ubiquitin7 is used as the housekeeping gene. Plotted values and error bars are fold-change means ± s.d. of two biological replicates. Asterisks (*) represent statistical differences assessed by one-way ANOVA followed by Tukey post-hoc test (p < 0.05). (B) Heatmap showing RNAseq expression data of MADS12, FT1, GA2ox3, GA2x4, and GA2ox5 genes in apical buds from mid-winter f2 to mid-spring f1. (C) Schematic representation of MADS12 and GA2ox4 antagonist expression pattern during bud dormancy release.