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. 2021 May 25;12:651444. doi: 10.3389/fphar.2021.651444

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Copyright © 2021 Li, Yang, Yin, Ma, Zhang and Tao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of OI on CCl4-induced hepatic injury in mice. (A) Representative photographs of the whole liver were taken 24 h after CCl4 treatment. (B) Representative HE staining hepatic images of each group. The black border indicated the necrosis area. (C) The necrosis area was measured based on H&E hepatic sections from each group. (D,E) Serum ALT and AST levels of each group. (F) The liver/body weight of each group. (G) Representative TUNEL staining hepatic sections for necrotic cells in each group. (H) The quantification of positive TUNEL cells per field. Positive cells were counted in five visual fields per liver section from each group. Original magnification, ×200. Data are expressed as the mean ± SEM (n = 5 for each group); #p < 0.05 vs. control group; *p < 0.05 vs. CCl4-treated group.