REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-Microtubule-Associated Protein 2 (MAP2) rabbit polyclonal antibody; 1:500	Millipore	Cat# AB5622 Lot# 3279314
Anti-S-100 Protein, clone 15E2E2 mouse monoclonal antibody; 1:200	Millipore	Cat# MAB079-1 Lot# 3308288
Neu-N antigen chicken polyclonal anti-peptide antibody; 1:200	Aves Labs	Cat# NUN Lot# NUN7807981
Alexa Fluor® 488 AffiniPure Donkey Anti-Chicken IgY (IgG) (H+L); 1:500	Jackson ImmunoResearch	Cat# 703-545-155 Lot# 147805
Cy™2 AffiniPure Donkey Anti-Rabbit IgG (H+L); 1:500	Jackson ImmunoResearch	Cat# 711-225-152 Lot# 146036
DyLight™405 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific; 1:500	Jackson ImmunoResearch	Cat# 115-475-205 Lot# 123839
Biological samples
Mouse dorsal root ganglion tissue	Jackson Laboratory	C57BL/6, #000664
Mouse sciatic nerve tissue	Jackson Laboratory	C57BL/6, #000664
Chemicals, peptides, and recombinant proteins
Hoechst 33342	Invitrogen	Cat# H3570
Diethylpyrocarbonate (DEPC)	VWR	Cat# 97062-650
1× Phosphate-buffered saline	Corning	Cat# 21-040-CV
Potassium chloride (KCl)	Sigma-Aldrich	Cat# 60130
Potassium dihydrogen phosphate (KH2PO4)	Fisher Biotech	Cat# AA1159430
Sodium phosphate, dibasic, heptahydrate (Na2PO4)	Fisher Scientific	Cat# S373-500
Sodium chloride (NaCl)	Sigma-Aldrich	Cat# 71380
Sucrose	Sigma-Aldrich	Cat# S5016
Paraformaldehyde	Sigma-Aldrich	Cat# P6148
Urethane	Sigma	Cat# U2500
Ketamine (Zetamine)	MWI	Cat# 501072
Xylazine (AnaSed)	MWI	Cat# 003437
Acepromazine maleate	MWI	Cat# 501075
Triton-X100	Fisher Scientific	Cat# BP151-100
Ethyl Alcohol 200 Proof	Pharmco	Cat# 111000200
Optimal cutting temperature (OCT) compound	Fisher Healthcare	Cat# 23-730-571
ProlongTM Gold Antifade Mountant	Invitrogen	Cat# P36931
Normal donkey serum (NDS)	Jackson ImmunoResearch	Cat# 017-000-121
Opal 570	Akoya Biosciences	Cat# FP1488001KT
Critical commercial assays
RNAscope® Multiplex Fluorescent Reagent Kit v2	Advanced Cell Diagnostics	Cat# 323100
3-Plex Negative Control Probe	Advanced Cell Diagnostics	Cat# 320871
Probe diluent	Advanced Cell Diagnostics	Cat# 300041
RNAscope Probe, Ppib	Advanced Cell Diagnostics	Cat# 313911-C3
RNAscope Probe, Lin28a	Advanced Cell Diagnostics	Cat# 437121
Experimental models: Organisms/strains
C57BL/6J Mice 7–16 weeks, male or female	Jackson Laboratory	Stock# 000664
Software and algorithms
Zeiss ZEN imaging software	Zeiss	N/A
FISHQuant v3	Florian Mueller	https://bitbucket.org/muellerflorian/fish_quant/src/master/
MATLAB R2019a	MathWorks	https://www.mathworks.com/
Imaris	Oxford Instruments	https://imaris.oxinst.com/
SortCellSize	Meffert Lab	https://github.com/Meffert-Lab/SortCellSize
Other
Confocal microscope	Zeiss	LSM 800
Numerical aperture (NA) 1.4 objective, ×63	Zeiss	N/A
Zeiss™ Immersol™ Immersion Oil	Zeiss	12-624-66A
Microtome cryostat	Microm	HM505E
Edge-Rite Disposable Microtome Blades	Thermo Scientific	Cat# 4280L
Fine paint brush	N/A	N/A
Vacuum pump	N/A	N/A
Petri dish (100 mm × 15 mm)	Denville Scientific	Cat# 1156F15
0.2 Micron filter	Thermo Scientific	Cat# 565-0020
Conical tubes, 15 mL	Greiner Bio-One	Cat# 188 271
Conical tubes, 50 mL	VWR	Cat# 89039-656
Microscope cover glass	Fisher Scientific	Cat# 12-545-E
Rotary shaker	N/A	N/A
Autoclave	N/A	N/A
Heat and stir plate	N/A	N/A
Incubator	VWR Scientific	Model 1565
25G ×1 needle	BD	Cat# 305125
Perfusion peristaltic pump	N/A	N/A
Needle-nosed pliers	N/A	N/A
Tissue-embedding molds (22 mm × 22 mm), (Peel-A-Way Truncated T-12)	Polysciences	Cat# 18986
Superfrost Plus microscopic slides	VWR	Cat# 48311-703
Polysine adhesion slides	Electron Microscopy Sciences	Cat# 63412-01
Steamer	Oster	N/A
Thermometer	N/A	N/A
Hydrophobic barrier pen	Vector Laboratories	Cat# H-4000
Slide rack (staining jar)	Ted Pella	Cat# 432-1
RapidFISH Slide Hybridization Oven (HybEZ Oven)	Boekel Scientific	Cat# 240200
IHC dark humidity tray	Ted Pella	Cat# 21053
Tissue wipes	Kimtech	Cat# 34120
Glass Pasteur pipette	N/A	N/A
Dry ice	N/A	N/A