Figure 2.

The GSE.8E3 transductant recognizes HIP11 in the context of DQ8 and DQ8-trans. The GSE.8E3 TCR clonotype identified from a CD4 T cell in the islets of a T1D organ donor was expressed in 5KC T-hybridoma cells with an NFAT-reporter. The GSE.8E3 TCR transductant was cultured with or without various HIPs (10 μM) or a native proinsulin peptide (100 μM) in the presence of K562 cells expressing DQ8 (black bars) or DQ8-trans (gray bars). ZsGreen-1 expression was evaluated by flow cytometry. Each result is the mean for three independent experiments. Error bars indicate the standard error of the mean.