Figure 3.

Response of HIP11-reactive T cells to insulin C-peptide. (A, B) The GSE.8E3 TCR transductant was cultured with various concentrations of a HIP11 peptide or an insulin C-peptide sequence in the presence of K562 cells expressing DQ8 (A) or DQ8trans (B). ZsGreen-1 positivity was assessed by flow cytometry as a measure of activation. Data are representative of three independent experiments. (C) The E2 T cell clone was cultured with various concentrations of a HIP11 peptide or an insulin C-peptide sequence in the presence of an autologous EBV-transformed B cell line. After 48 hours, supernatants from T cell cultures were harvested and IFN-γ concentrations were measured by ELISA. Results are representative of two independent experiments.