Figure 5.

Effect of (+)-PTZ on WT ONHAs migration after five hours of treatment and on lamellipodia marker expression. WT ONHAs were pretreated with 10 µM (+)-PTZ or vehicle for one hour before scratching. After scratching, the cells were incubated with 10 µM (+)-PTZ or vehicle for five hours. (A) Representative images show the original scratch (day 0), and migration of WT ONHAs into the wound area after five hours of incubation with (+)-PTZ treatment or vehicle control. Scale bar: 200 µm. (B) Wound distance was measured using ZEN software. The wound distance significantly decreased when WT ONHAs were treated with (+)-PTZ. Significantly different from control **P < 0.01. The results were repeated in triplicate with cells isolated from different animals, on different dates, and treated on different days. (C) Representative images show cells stained with Arp3 antibody (red), phalloidin (green), and DAPI (blue). Scale bar: 50 µm. (D) Quantification of the number of lamellipodia for each cell. For each group, 100 cells, located at the wound edge, were quantified. (+)-PTZ treated cells had significantly more lamellipodia than control ONHAs. Significantly different from control **P < 0.01. Data were analyzed using Student's t-test. The results were repeated in triplicate with cell cultures isolated from different animals, on different dates, and treated on different days.