Figure 8.

Effect of (+)-PTZ on migration of OGD/R-exposed ONHAs. Both WT and S1R KO ONHAs were pretreated with 10 µM (+)-PTZ for one hour before scratching. After scratching, the cells were incubated with 10 µM (+)-PTZ under OGD for six hours, followed by reoxygenation for 18 hours. (A) Representative images show migration of WT versus S1R KO normoxic (control) and OGD/R-exposed cells into the wounded area with or without (+)-PTZ treatment. Scale bar: 200 µm. (B) Cells in wounded area were counted by ImageJ. For each group, four coverslips were quantified. WT ONHA migration increased when cells were treated with (+)-PTZ after OGD/R. Significantly different from control ****P < 0.0001. S1R KO ONHA migration was not affected by (+)-PTZ treatment. Data were analyzed using one-way ANOVA followed by Tukey-Kramer post hoc test for multiple comparisons. Experiments were repeated in triplicates with cells isolated from different dates, different animals and treated on different days. For each group of each isolation, three coverslips were quantified.