Figure 9.

Effect of (+)-PTZ on phosphorylation of STAT3 and NFkB p-65 in WT ONHAs. (A) Representative Western blot showing increased phosphorylation of both STAT3 and NFkB p-65 after OGD/R in WT ONHAs. (+)-PTZ treatment enhanced OGD/R-induced STAT-3 phosphorylation, whereas (+)-PTZ treatment abrogated OGD/R-induced phosphorylation of p-65. In addition, S1R expression was increased under OGD/R exposure. (B–D) Quantitative analysis by ImageJ. Significantly different from control **P < 0.01, *P < 0.05. Data were analyzed using one-way ANOVA followed by Tukey-Kramer for multiple comparisons. These experiments were repeated in triplicate with cells isolated from different dates, different animals and treated on different days.