Figure 6.
Cell-cycle analysis highlights progenitor characteristics of eFGF2-treated cultures
(A) Quantification method for determining the cleavage angle of dividing PH3+ progenitors.
(A′) Images showing horizontal (filled arrows) or vertical (hollow arrows) cleavage angles (n = 103 cells).
(B) Quantification of cleavage angle under Basal, eFGF, and MEKi conditions. eFGF2-treated progenitors preferentially underwent proliferative divisions, shown by the increase in vertical cleavage planes.
(C) EdU assessment of cell-cycle kinetics with EdU labeling cells in S phase and Ki67 total cycling pool. Inset shows magnified region. Note the two PH3+ mitotic progenitors are EdU− at this time point (4 h), showing that they had exited S phase prior to EdU addition.
(D) Representation of G1, S, G2, and M phase and total cell-cycle length under Basal, eFGF2, or MEKi conditions, highlighting that eFGF2 shortened the cell cycle (representative of n = 3 independent experiments).
Scale bar, 50 μm.