Figure 2.

MTG staining reveals heterogeneity in X1 cells

(A) Dot plot showing the FACS gating for X1-MTG^Low and MTG^High cells.

(B) PIWI-1 immunostaining in FACS sorted X1-MTG^Low and MTG^High cells quantified for PIWI-1^High through flow cytometry. Mean ± SEM, over three independent replicates. ^∗Represents p < 0.05. See also Figure S1.

(C) Representative confocal images of PIWI-1 staining in indicated sorted population. PIWI-1 staining is shown using Fire LUT (ImageJ). Arrowheads highlight PIWI-1 low (yellow) and negative (red) cells. Scale bar, 30 μm.

(D) Volcano plot displaying differential gene expression between X1-MTG^Low and MTG^High cells.

(E) Bar graph representing selected GO terms that are downregulated in X1-MTG^Low cells compared with MTG^High. Refer to Table S1 for the full list of GO terms.

(F) Heatmap indicating the expression of the differentially expressed genes in X1-MTG^Low vs MTG^High cells in the published single-cell database (Zeng et al., Cell 2018).