Figure 1.

Temporal dynamics of protein and gene expression along the time course of 2D micropatterned gastruloid BMP4 treatment

(A and B) Immunofluorescence images of (A) SOX2, NANOG, and POU5F1 and (B) CDH1, T, CDH2, TFAP2A, SOX17, and GATA3 at the indicated time points. Scale bar, 100 μm.

(C and D) Quantification of normalized fluorescence intensity of (C) SOX2, NANOG, POU5F1, T, SOX17, and CDH2 and (D) TFAP2A and GATA3 after 12, 24, and 44 h of BMP4 treatment. Number of experiments, n = 1 for T, SOX17, CDH2, GATA3, and TFAP2A at 12 and 24 h; n = 1 for TFAP2A at 44 h; n = 2 for GATA3 at 44 h; n = 3 for SOX2, NANOG, and POU5F1 at 12, 24, and 44 h; n = 5 for T and CDH2 at 44 h; n = 6 for SOX17 at 44 h. Data are presented as the mean ± SEM.

(E) Uniform manifold approximation and projection (UMAP) display of indicated marker expression at indicated time points.