Figure 3.

Comparison of the 2D micropatterned gastruloid cell types with the human Carnegie stage 7 gastrula

(A and B) Comparison of CS7 gastrula with BMP4 micropatterned cultures at the indicated time points using (A) average gene expression correlation and (B) anchor-based prediction.

(C) Prediction score of CS7 cell types in the indicated gastruloid cell types.

(D) Violin plot of the PGC module score in gastruloid cell types.

(E and F) Prediction scores of mouse ectodermal derivatives in ectoderm of (E) CS7 and (F) gastruloid.

(G) Expression of indicated ectoderm makers in the gastruloid ectoderm cluster.